PRELIMINARY CRYSTALLOGRAPHIC STUDY OF PEANUT PEROXIDASE

被引：12

作者：

BAN, N

VANHUYSTEE, RB

DAY, J

GREENWOOD, A

LARSON, S

ESNAULT, R

MCPHERSON, A

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

[2] UNIV WESTERN ONTARIO,DEPT PLANT SCI,LONDON N6A 4B7,ONTARIO,CANADA

[3] CNRS,INST SCI VEGETALES,F-91198 GIF SUR YVETTE,FRANCE

来源：

ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE | 1992年 / 48卷

关键词：

D O I：

10.1107/S0108768191008807

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The cationic isozyme of peroxidase isolated from suspension cultures of peanut cells is a heme-containing and calcium-dependent glycoprotein having four covalently attached oligosaccharide chains. Attempts were made to crystallize the glycoprotein for X-ray diffraction analysis, and these have met with some success. Crystals have now been grown that are suitable for a full three-dimensional structural analysis. The crystals are thin plates and we have shown them to be of the orthorhombic space group P2(1)2(1)2(1) with a = 48.1, b = 97.2, c = 146.2 angstrom. The crystals diffract to beyond 2.8 angstrom resolution, appear to be stable to lengthy X-ray exposure, and contain two molecules of 40 000 daltons each in the asymmetric unit.

引用

页码：109 / 111

页数：3

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