THE ALKALINE-PHOSPHATASE IN HUMAN PLEXUS CHORIOIDEUS

被引:10
作者
NISHIHARA, Y
HAYASHI, Y
FUJII, T
ADACHI, T
STIGBRAND, T
HIRANO, K
机构
[1] GIFU PHARMACEUT UNIV,DEPT PHARMACEUT,GIFU 502,JAPAN
[2] UMEA UNIV,DEPT MED BIOCHEM & BIOPHYS,S-90187 UMEA,SWEDEN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1209卷 / 02期
关键词
ALKALINE PHOSPHATASE; ISOZYME; (HUMAN BRAIN);
D O I
10.1016/0167-4838(94)90196-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The content of alkaline phosphatase isozymes in various brain regions was determined by monoclonal immunocatalytic assays. The levels of the isozymes in human brain tissues were low compared with those in other human tissues, liver, kidney, bone, intestine and placenta. Plexus chorioideus in the brain, however, was found to express significant amounts of alkaline phosphatase activity. The purified isozyme from human plexus chorioideus demonstrated a single 70 kDa protein band on SDS-polyacrylamide gel which coincides with that of tissue-unspecific alkaline phosphatase from human liver. The isozyme expressed in the plexus was confirmed to be the tissue-unspecific alkaline phosphatase isozyme with regard to its reactivity with monoclonal antibodies specific for liver alkaline phosphatase, heat stability, and the inhibition by amino acids. This finding adds new dimensions to the functional role this isozyme may play.
引用
收藏
页码:274 / 278
页数:5
相关论文
共 25 条
[1]  
CHATTERJEE TK, 1979, INDIAN J BIOCHEM BIO, V16, P268
[2]   ULTRASTRUCTURAL LOCALIZATION OF ALKALINE-PHOSPHATASE IN CULTURES OF NERVOUS-TISSUE IN-VITRO [J].
CIANI, F ;
CONTESTABILE, A ;
MINELLI, G ;
QUAGLIA, A .
JOURNAL OF NEUROCYTOLOGY, 1973, 2 (02) :105-116
[3]   IMMUNOLOGICAL IDENTITY OF URINARY ALKALINE-PHOSPHATASE IN HEALTH AND DISEASE [J].
CORNELL, A ;
HODSON, AW ;
LATNER, AL .
CLINICA CHIMICA ACTA, 1979, 97 (2-3) :217-223
[4]   EUKARYOTIC PROTEIN MODIFICATION AND MEMBRANE ATTACHMENT VIA PHOSPHATIDYLINOSITOL [J].
CROSS, GAM .
CELL, 1987, 48 (02) :179-181
[5]   PURIFICATION AND PROPERTIES OF BRAIN ALKALINE-PHOSPHATASE [J].
DORAI, DT ;
BACHHAWAT, BK .
JOURNAL OF NEUROCHEMISTRY, 1977, 29 (03) :503-512
[6]   PERSPECTIVES ON ALKALINE-PHOSPHATASE ISOENZYMES [J].
FISHMAN, WH .
AMERICAN JOURNAL OF MEDICINE, 1974, 56 (05) :617-650
[7]   MAMMALIAN BRAIN ALKALINE-PHOSPHATASE - EXPRESSION OF LIVER-BONE-KIDNEY LOCUS - COMPARISON OF FETAL AND ADULT ACTIVITIES [J].
GOLDSTEIN, DJ ;
HARRIS, H .
JOURNAL OF NEUROCHEMISTRY, 1981, 36 (01) :53-57
[8]  
HAYASHI Y, 1991, JPN J CLIN CHEM, V20, P125
[9]   SPECIFIC ASSAYS FOR HUMAN ALKALINE-PHOSPHATASE ISOZYMES [J].
HIRANO, K ;
MATSUMOTO, H ;
TANAKA, T ;
HAYASHI, Y ;
IINO, S ;
DOMAR, U ;
STIGBRAND, T .
CLINICA CHIMICA ACTA, 1987, 166 (2-3) :265-273
[10]   A HIGHLY SENSITIVE ASSAY-METHOD FOR HUMAN PLACENTAL ALKALINE-PHOSPHATASE INVOLVING A MONOCLONAL-ANTIBODY BOUND TO A PAPER DISK [J].
HIRANO, K ;
IIZUMI, Y ;
HAYASHI, Y ;
TANAKA, T ;
SUGIURA, M ;
HAYASHI, K ;
LU, ZD ;
IINO, S .
ANALYTICAL BIOCHEMISTRY, 1986, 154 (02) :624-631