SPECIFIC PROLINE AND HYDROXYPROLINE DETECTION METHOD BY POSTCOLUMN DERIVATIZATION FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10

作者：

OZAKI, A ^{[1
]}

SHIBASAKI, T ^{[1
]}

MORI, H ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS, MACHIDA 194, TOKYO, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 09期

关键词：

D O I：

10.1271/bbb.59.1764

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific detection method for hydroxyproline by post-column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole was developed after chiral resolution by ligand-exchange chromatography, The addition of ethylenediaminetetraacetic acid to the reaction mixture was effective to achieve the post-column reaction, The calibration curve for trans-4-hydroxy-L-proline shows linearity from 7.6 pmol up to 7600 pmol.

引用

页码：1764 / 1765

页数：2

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