EFFECTS OF ELUENT COMPOSITION, ION-PAIR REAGENT AND TEMPERATURE ON THE SEPARATION OF HISTONES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

LINDNER, H

HELLIGER, W

机构：

[1] Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Innsbruck, A-6020

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 9-10期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; HISTONES; HMG PROTEINS; MOBILE PHASE MODIFIERS; TEMPERATURE EFFECT; ION-PAIR EFFECT;

D O I：

10.1007/BF02269797

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the eluent composition, ion-pair concentration, and temperature on the fractionation of chromosomal proteins by reversed-phase high performance liquid chromatography (HPLC) has been investigated. Based on this examination methods for the troublesome separation of histones H1-degrees/H1b, H2A/H4, and H5.1/H5.2 have been developed.

引用

收藏

页码：518 / 522

页数：5

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