EPIZOOTIC HEMATOPOIETIC NECROSIS VIRUS - DETECTION BY ELISA, IMMUNOHISTOCHEMISTRY AND IMMUNOELECTRON-MICROSCOPY

Epizootic haematopoietic necrosis virus (EHNV) was isolated from cultured rainbow trout, Oncorhynchus mykiss (Walbaum). Antibodies to the virus and to associated capsid subunits have been produced and used in immunohistochemistry, immunoelectron-microscopy and an antigen-capture-immunosorbent assay (ELISA). The results show that both antibodies can be used by various immuno-procedures to detect both redfin perch, Perca fluviatilis L., and rainbow trout isolates of EHNV. The procedures described provide for the first time rapid and specific tests for the detection of EHNV in cultured and clinical material.