A C-13 NMR-STUDY ON FLUXES INTO THE TCA CYCLE OF NEURONAL AND GLIAL TUMOR-CELL LINES AND PRIMARY-CELLS

被引：59

作者：

BRAND, A ^{[1
]}

ENGELMANN, J ^{[1
]}

LEIBFRITZ, D ^{[1
]}

机构：

[1] UNIV BREMEN,FACHBEREICH 2,W-2800 BREMEN 33,GERMANY

来源：

BIOCHIMIE | 1992年 / 74卷 / 9-10期

关键词：

TCA CYCLE; NEURON AND GLIA CELLS; H-1 AND C-13 NMR; PYRUVATE CARBOXYLASE; PYRUVATE DEHYDROGENASE ACTIVITIES;

D O I：

10.1016/0300-9084(92)90078-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-C-13-pyruvate and 3-C-13-pyruvate in culture dishes. C-13 NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism.

引用

页码：941 / 948

页数：8

共 21 条

[1] CEREBRAL METABOLISM OF ACETATE AND GLUCOSE STUDIED BY C-13-NMR SPECTROSCOPY - A TECHNIQUE FOR INVESTIGATING METABOLIC COMPARTMENTATION IN THE BRAIN [J].