THE CARDIAC TROPONIN-T ALTERNATIVE EXON CONTAINS A NOVEL PURINE-RICH POSITIVE SPLICING ELEMENT

被引:196
作者
XU, RS
TENG, J
COOPER, TA
机构
[1] BAYLOR COLL MED, DEPT PATHOL, 1 BAYLOR PL, HOUSTON, TX 77030 USA
[2] BAYLOR COLL MED, DEPT CELL BIOL, HOUSTON, TX 77030 USA
关键词
D O I
10.1128/MCB.13.6.3660
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have characterized a novel positive-acting splicing element within the developmentally regulated alternative exon (exon 5) of the cardiac troponin T (cTNT) gene. The exon splicing element (ESE) is internal to the exon portions of the splice sites and is required for splicing to the 3' splice site but not the 5' splice site flanking the exon. Sequence comparisons between cTNT exon 5 and other exons that contain regions required for splicing reveal a common purine-rich motif. Sequence within cTNT exon 5 or a synthetic purine-rich motif facilitates splicing of heterologous alternative and constitutive splice sites in vivo. Interestingly, the ESE is not required for the preferential inclusion of cTNT exon 5 observed in primary skeletal muscle cultures. Our results strongly suggest that the purine-rich ESE serves as a general splicing element that is recognized by the constitutive splicing machinery.
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页码:3660 / 3674
页数:15
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