TIME-RESOLVED W-BAND (95 GHZ) EPR SPECTROSCOPY OF ZN-SUBSTITUTED REACTION CENTERS OF RHODOBACTER-SPHAEROIDES R-26

被引：102

作者：

PRISNER, TF ^{[1
]}

VANDEREST, A ^{[1
]}

BITTL, R ^{[1
]}

LUBITZ, W ^{[1
]}

STEHLIK, D ^{[1
]}

MOBIUS, K ^{[1
]}

机构：

[1] TECH UNIV BERLIN,MAX VOLMER INST,D-10623 BERLIN,GERMANY

来源：

CHEMICAL PHYSICS | 1995年 / 194卷 / 2-3期

关键词：

D O I：

10.1016/0301-0104(95)00016-H

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Transient EPR spectra of protonated and deuterated Zn-substituted reaction centres of Rhodobacter sphaeroides R-26, measured at a microwave frequency of 95 GHz and an external magnetic field of 3.5 T, are presented. The high-field/high-frequency spin-polarized spectrum of the correlated coupled radical pair (P865OA-.)-O-+. after laser flash excitation is a very sensitive monitor of the relative orientation of the two g-matrices and the dipolar tenser with respect to each other. Therefore, detailed structural information of the RC concerning the relative orientation of the primary donor P-856(+.) and the acceptor Q(A)(-.) with respect to the axis connecting the two molecules in the RC can be derived. Together with the information obtained by high-field cw-EPR on single crystals, the enhanced resolution of the polarized high-field spectra allows an unambiguous assignment of the g-matrix of the donor P-865(+.) to the molecular axis system. The experimental results are compared with earlier X-band (9 GHz) and K-band (24 GHz) EPR experiments.

引用

页码：361 / 370

页数：10

共 34 条

[1] TIME-RESOLVED EPR OF THE RADICAL PAIR P(865)(+)Q(A)(-) IN BACTERIAL REACTION CENTERS - OBSERVATION OF TRANSIENT NUTATIONS, QUANTUM BEATS AND ENVELOPE MODULATION EFFECTS [J].

BITTL, R ;

VANDEREST, A ;

KAMLOWSKI, A ;

LUBITZ, W ;

STEHLIK, D .

CHEMICAL PHYSICS LETTERS, 1994, 226 (3-4) :349-358

[2] TRANSIENT EPR OF RADICAL PAIRS IN PHOTOSYNTHETIC REACTION CENTERS - PREDICTION OF QUANTUM BEATS [J].

BITTL, R ;

KOTHE, G .

CHEMICAL PHYSICS LETTERS, 1991, 177 (06) :547-553

[3]

BOCK CH, 1991, THESIS FU BERLIN

[4] 3-MM HIGH-FIELD EPR ON SEMIQUINONE RADICAL-ANIONS Q.- RELATED TO PHOTOSYNTHESIS AND ON THE PRIMARY DONOR P.+ AND ACCEPTOR QA.- IN REACTION CENTERS OF RHODOBACTER-SPHAEROIDES R-26 [J].

BURGHAUS, O ;

PLATO, M ;

ROHRER, M ;

MOBIUS, K ;

MACMILLAN, F ;

LUBITZ, W .

JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (29) :7639-7647

[5] STRUCTURE OF THE MEMBRANE-BOUND PROTEIN PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER-SPHAEROIDES [J].

CHANG, CH ;

ELKABBANI, O ;

TIEDE, D ;

NORRIS, J ;

SCHIFFER, M .

BIOCHEMISTRY, 1991, 30 (22) :5352-5360

[6] SPIN-POLARIZED ELECTRON-PARAMAGNETIC RESONANCE-SPECTRA OF RADICAL PAIRS IN MICELLES - OBSERVATION OF ELECTRON-SPIN SPIN INTERACTIONS [J].

CLOSS, GL ;

FORBES, MDE ;

NORRIS, JR .

JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (13) :3592-3599

[7]

ERMLER U, 1992, RES PHOTOSYNTHESIS, V1, P341

[8] TRANSIENT EPR SPECTROSCOPY OF THE CHARGE SEPARATED STATE P+Q- IN PHOTOSYNTHETIC REACTION CENTERS - COMPARISON OF ZN-SUBSTITUTED RHODOBACTER-SPHAEROIDES R-26 AND PHOTOSYSTEM-I [J].

FUCHSLE, G ;

BITTL, R ;

VANDEREST, A ;

LUBITZ, W ;

STEHLIK, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1142 (1-2) :23-35

[9] ELECTRON-SPIN POLARIZATION OF PHOTOSYNTHETIC REACTANTS [J].

HOFF, AJ .

QUARTERLY REVIEWS OF BIOPHYSICS, 1984, 17 (02) :153-282

[10]

Hore P. J., 1989, ADV EPR APPL BIOL BI, P405

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