IDENTIFICATION OF N-TERMINUS PEPTIDE OF HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR AS THE SITE OF NUCLEOTIDE INTERACTION

被引：4

作者：

CHAVAN, AJ ^{[1
]}

GASS, C ^{[1
]}

HALEY, BE ^{[1
]}

BOONE, T ^{[1
]}

DOUKAS, MA ^{[1
]}

机构：

[1] UNIV KENTUCKY,COLL PHARM,VET ADM MED CTR,LUCILLE P MARKEY CANC CTR,LEXINGTON,KY 40536

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1350

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of nucleotides with recombinant human granulocyte/macrophage colony stimulating factor (rhGM-CSF) has been investigated. Utilizing nucleotide photoaffinity probes [gamma(32)P]-8N(3)ATP and [beta'P-32]-8N(3)Ap(4)A, an analog of alarmone, the specificity of interaction was demonstrated by saturation of photoinsertion by these analogs and protection of photoinsertion by these analogs in the presence of natural nucleotide. The site of photoinsertion was tentatively determined to be Ser(9). The photolabeled cytokine has lost most of its biological activity in a cellular proliferation assay, indicating a possible physiological role for this interaction. (C) 1995 Academic Press. Inc.

引用

页码：390 / 396

页数：7

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