THE NITRATE REDUCTASE GENE FROM A SHOYU KOJI MOLD, ASPERGILLUS-ORYZAE KBN616

被引：42

作者：

KITAMOTO, N

KIMURA, T

KITO, Y

OHMIYA, K

TSUKAGOSHI, N

机构：

[1] MIE UNIV, FAC BIORESOURCES, TSU, MIE 504, JAPAN

[2] NAGOYA UNIV, SCH AGR, DEPT APPL BIOL SCI, NAGOYA, AICHI 464, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 09期

关键词：

D O I：

10.1271/bbb.59.1795

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A niaD gene encoding nitrate reductase was isolated from Aspergillus oryzae KBN616 and sequenced. The structural gene comprises 2973 bp and 868 amino acids, which showed a high degree of similarity to nitrate reductases from other filamentous fungi. The coding sequence is interrupted by six introns varying in size from 48 to 98 bp. The intron positions are all conserved among the niaD genes from A. oryzae, Aspergillus nidulans, and Aspergillus niger. A homologous transformation system was developed for an industrial shoyu koji mold, A. oryzae KBN616, based on the nitrate reductase (niaD) of the nitrate assimilation pathway.

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收藏

页码：1795 / 1797

页数：3

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