DETERMINATION OF D-MYO-1,2,6-INOSITOL TRISPHOSPHATE BY ION-PAIR REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH POST-COLUMN LIGAND-EXCHANGE AND FLUORESCENCE DETECTION

被引：26

作者：

IRTH, H ^{[1
]}

LAMOREE, M ^{[1
]}

DEJONG, GJ ^{[1
]}

BRINKMAN, UAT ^{[1
]}

FREI, RW ^{[1
]}

KORNFELDT, RA ^{[1
]}

PERSSON, L ^{[1
]}

机构：

[1] PERSTORP PHARMA,S-28480 PERSTORP,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 499卷

关键词：

D O I：

10.1016/S0021-9673(00)97006-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A detection system for d-myo-1,2,6-inositol trisphosphate (1,2,6-IP3) has been developed which is based on the strong complexing properties of the phosphate groups and which includes a post-column ligand-exchange reaction. The weakly fluorescent complex of FeIII with methylcalcein blue (MCB) is used as a reagent. Analytes that form a stronger complex with FeIII than MCB cause a ligand-exchange reaction; the amount of strongly fluorescent MCB released during this reaction is proportional to the analyte concentration and can be measured sensitively by direct fluorescence detection. The influence of the pH, the reagent concentration, the FeIII to MCB ratio, the content of organic modifier and the dimensions of the post-column reaction system on the sensitivity of the detection system are discussed. In combination with ion-pair reversed-phase liquid chromatography, a detection limit of 3-10 ng has been obtained for 1,2,6-IP3. The present system has been combined with a preconcentration technique, based on ion-pair formation, which allows the determination of lo-ppb concentrations of 1,2,6-IP3. © 1990.

引用

页码：617 / 625

页数：9

共 22 条

[1]

AUTHI KS, 1989, THROMB HAEMOSTASIS, V62, P250

[2]

Berridge M.J., 1985, Scientific American, V253, P124

[3] SEPARATION OF INOSITOL PHOSPHATES AND GLYCEROPHOSPHOINOSITOL PHOSPHATES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].