LONG-TERM CULTURES OF CHICKEN BONE-MARROW CELLS

被引:9
作者
CORMIER, F
DIETERLENLIEVRE, F
机构
[1] Institut d'Embryologie Cellulaire et Moléculaire, 49bis, avenue de la Belle Gabrielle
关键词
D O I
10.1016/0014-4827(90)90151-Y
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We report an adaptation to cultures of chicken bone marrow cells of the Dexter culture technique for obtaining long-term hemopoiesis in vitro. Cells were seeded in DMEM supplemented with fetal calf serum (20%) and hydrocortisone (10-6 M) with or without chicken serum (1%). Cultures were incubated at 37 °C and fed every 2 weeks. An adherent cell layer composed of macrophages, flbroblasts, and adipocytes became established, over which hemopoietic cells formed foci and were released into the supernatant. Granulocytes and monocytes-macrophages differentiated in a constant proportion until Week 6, whereafter differentiation became progressively restricted to the monocytic lineage. As demonstrated by the generation of colony-forming cells, hemopoiesis was maintained for either 12 or 28 weeks. © 1990.
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页码:113 / 117
页数:5
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