INTERNAL ELIMINATED SEQUENCES ARE REMOVED PRIOR TO CHROMOSOME FRAGMENTATION DURING DEVELOPMENT IN EUPLOTES-CRASSUS

被引：43

作者：

TAUSTA, SL

KLOBUTCHER, LA

机构：

[1] Department of Biochemistry, University of Connecticut Health Center, Farmington

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 04期

关键词：

D O I：

10.1093/nar/18.4.845

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hypotrichous ciliated protozoa undergo a massive genome rearrangement process after their sexual cycle. One frequent type of rearrangement is the removal of DNA sequences (internal eliminated sequences; IESs) from internal regions of DNA molecules. In this study, we characterized the removal of IESs in Euplotes crassus. Southern hybridization analyses combined with cytological observations Indicated that IES removal is an early event in macronuclear development, occurring during the polytene chromosome stage and prior to the chromosome fragmentation process. The results are consistent with IES removal occurring via an intramolecular DNA breakage and rejoining process. © 1990 Oxford University Press.

引用

页码：845 / 853

页数：9

共 39 条

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