学术探索
学术期刊
新闻热点
数据分析
智能评审

EXPRESSION OF TRANSLATION INITIATION FACTOR-4A FROM YEAST AND MOUSE IN SACCHAROMYCES-CEREVISIAE

被引：26
作者：
PRAT, A
SCHMID, SR
BUSER, P
BLUM, S
TRACHSEL, H
NIELSEN, PJ
LINDER, P
机构：
[1] BIOZENTRUM,DEPT MICROBIOL KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND
[2] UNIV BERN,INST BIOCHEM & MOLEK BIOL,CH-3000 BERN,SWITZERLAND
[3] MAX PLANCK INST IMMUNBIOL,W-7800 FREIBURG,GERMANY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1050卷 / 1-3期
关键词：
(S. cerevisiae); CAP binding complex; Conditional expression; Helicase; Mutation; Temperature sensitive mutation;
D O I：
10.1016/0167-4781(90)90155-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The eukaryotic translation initiation factor 4A (eIF-4A) plays an important role in regulating initiation. To analyze its function in yeast, we carried out a mutational analysis of the TIF1 and TIF2 genes, which encode eIF-4A. Expression of these two yeast genes has also been investigated at the transcriptional level and it has been found that both are expressed in wild-type yeast cells. Analysis of the expression of eIF-4A-β-galactosidase fusion proteins reveals that the TIF2 gene is more highly expressed than the TIF1 gene. Interestingly, the yeast eIF-4A protein shows a high degree of amino acid sequence similarity to the mouse homologue. However, we find that the mammalian factor does not support protein synthesis in yeast either in vivo or in vitro. © 1990.
引用
收藏
页码：140 / 145
页数：6
相关论文
共 34 条
[1]  
ALTMANN M, 1989, J BIOL CHEM, V264, P12145
[2]   MESSENGER-RNA STRUCTURES INFLUENCING TRANSLATION IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].
BAIM, SB ;
SHERMAN, F .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (04) :1591-1601
[3]   TRANSLATION IN SACCHAROMYCES-CEREVISIAE - INITIATION-FACTOR 4A-DEPENDENT CELL-FREE SYSTEM [J].
BLUM, S ;
MUELLER, M ;
SCHMID, SR ;
LINDER, P ;
TRACHSEL, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6043-6046
[4]  
CASADABAN MJ, 1983, METHOD ENZYMOL, V100, P293
[5]   IDENTIFICATION OF 5 PUTATIVE YEAST RNA HELICASE GENES [J].
CHANG, TH ;
ARENAS, J ;
ABELSON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1571-1575
[6]   AN ARS SILENCER BINDING-FACTOR ALSO ACTIVATES 2 RIBOSOMAL-PROTEIN GENES IN YEAST [J].
DORSMAN, JC ;
DOORENBOSCH, MM ;
MAURER, CTC ;
DEWINDE, JH ;
MAGER, WH ;
PLANTA, RJ ;
GRIVELL, LA .
NUCLEIC ACIDS RESEARCH, 1989, 17 (13) :4917-4923
[7]  
DUNCAN R, 1983, J BIOL CHEM, V258, P7228
[8]   FUNCTIONAL SUBSTITUTION OF MOUSE RIBOSOMAL PROTEIN-L27' FOR YEAST RIBOSOMAL PROTEIN-L29 IN YEAST RIBOSOMES [J].
FLEMING, G ;
BELHUMEUR, P ;
SKUP, D ;
FRIED, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :217-221
[9]   ATP-BINDING SITE OF ADENYLATE KINASE - MECHANISTIC IMPLICATIONS OF ITS HOMOLOGY WITH RAS-ENCODED P21, F1-ATPASE, AND OTHER NUCLEOTIDE-BINDING PROTEINS [J].
FRY, DC ;
KUBY, SA ;
MILDVAN, AS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (04) :907-911
[10]  
GOYER C, 1989, J BIOL CHEM, V264, P7603
1234