PREPARATION AND CHARACTERIZATION OF MN SALOPHEN COMPLEX WITH SUPEROXIDE SCAVENGING ACTIVITY

被引:36
作者
LIU, ZX [1 ]
ROBINSON, GB [1 ]
GREGORY, EM [1 ]
机构
[1] VIRGINIA POLYTECH INST & STATE UNIV, DEPT BIOCHEM & ANAEROB MICROBIOL, BLACKSBURG, VA 24061 USA
关键词
SUPEROXIDE DISMUTASE-LIKE ACTIVITY; LOW MOLECULAR WEIGHT; SUPEROXIDE; SUPEROXIDE DISMUTASE MIMIC; FREE RADICALS; OXYGEN TOXICITY;
D O I
10.1006/abbi.1994.1473
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mn(III)-salophen complex with superoxide scavenging activity was prepared from manganese(III) acetate dihydrate and salophen in ethanol. Visible absorption spectrum of the red-brown complex exhibits a shoulder at 430 nm which was absent with either salophen or manganic acetate alone. Titration of salophen with manganese(III) is consistent with a 1:1 Mn to salophen stoichiometry of the complex based on changes in the absorbance at 500 nm or of superoxide scavenging activity. The superoxide dismutase (SOD)-like activity of the complex in the xanthine-xanthine oxidase/cytochrome c assay was 1450 units/mg salophen. The SOD activity of the complex was suppressed 50% in the presence of EDTA (1 mM), but was not altered in the presence of bovine serum albumin (1 mg/ml) or crude protein extract of Escherichia coli QC779 sodA(-) sodB(-) (1 mg/ml). E. coli QC779 sodA(-) sodB(-) grew scantily after an 8-h lag phase in aerobic M63 glucose minimal medium. The aerobic growth of the E. coli SOD double mutant in glucose minimal medium was greatly enhanced in the presence of 5 or 10 mu M Mn-salophen complex compared to that of control after 24 h incubation. Mn-desferal green complex (10 mu M) and pink complex (5 mu M) also increased growth rate of E. coli QC779 sodA(-) sodB(-) but to a lesser extent than Mn-salophen complex. However, the growth was completely inhibited by 50 mu M Mn-salophen complex, 100 mu M Mn-desferal green complex, or 10 mu M Mn-desferal pink complex. (C) 1994 Academic Press, Inc.
引用
收藏
页码:74 / 81
页数:8
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