CHEMICAL-ANALYSIS OF NEUROTRANSMITTER CANDIDATES IN CLONAL CELL-LINES FROM DROSOPHILA CENTRAL-NERVOUS-SYSTEM .1. ACH AND L-DOPA

被引：14

作者：

UITEI, K

NISHIHARA, S

SAKUMA, M

MATSUDA, K

MIYAKE, T

MIYATA, Y

机构：

[1] NIPPON MED COLL,DEPT PHARM,BUNKYO KU,TOKYO 113,JAPAN

[2] MITSUBISHI KASEI INST LIFE SCI,DEPT BIOL MOLEC,MACHIDA 194,TOKYO,JAPAN

来源：

NEUROSCIENCE LETTERS | 1994年 / 174卷 / 01期

关键词：

NEUROTRANSMITTER; ACETYLCHOLINE; CATECHOLAMINE; L-DOPA; SEROTONIN; OCTOPAMINE; DROSOPHILA; CNS CELL LINE;

D O I：

10.1016/0304-3940(94)90125-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To characterize neuronal phenotypes, aminergic neurotransmitters, i.e. acetylcholine (ACh), catecholamine (CA)s and other biogenic amines (serotonin, octopamine), were surveyed in 10 colonial clones from one cell line of Drosophila larval CNS using HPLC-ECD system. ACh, which is a neurotransmitter in Drosophila, was found in 7 out of 10 clones. CAs, their metabolites, and other amines were not detected in any clones. However, all the 10 clones expressed L-DOPA, a precursor of CAs. Consequently, seven clones expressed ACh and L-DOPA. L-DOPA as a novel neurotransmitter candidate was discussed.

引用

收藏

页码：85 / 88

页数：4

相关论文

共 24 条

[1]

ARAKAWA S, 1990, NEURON, V2, P343

[2] CATECHOLAMINE-CONTAINING NEURONS IN DROSOPHILA-MELANOGASTER - DISTRIBUTION AND DEVELOPMENT [J].

BUDNIK, V ;

WHITE, K .

JOURNAL OF COMPARATIVE NEUROLOGY, 1988, 268 (03) :400-413

[3]

BUDNIK V, 1986, J NEUROSCI, V6, P3682

[4] TRANSMITTER-LIKE RELEASE OF ENDOGENOUS 3,4-DIHYDROXYPHENYLALANINE FROM RAT STRIATAL SLICES [J].

GOSHIMA, Y ;

KUBO, T ;

MISU, Y .

JOURNAL OF NEUROCHEMISTRY, 1988, 50 (06) :1725-1730

[5] NANOMOLAR L-DOPA FACILITATES RELEASE OF DOPAMINE VIA PRESYNAPTIC BETA-ADRENOCEPTORS - COMPARATIVE-STUDIES ON THE ACTIONS IN STRIATAL SLICES FROM CONTROL AND 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE (MPTP)-TREATED C57 BLACK MICE, AN ANIMAL-MODEL FOR PARKINSONS-DISEASE [J].

GOSHIMA, Y ;

MISU, Y ;

ARAI, N ;

MISUGI, K .

JAPANESE JOURNAL OF PHARMACOLOGY, 1991, 55 (01) :93-100

[6] L-DOPA FACILITATES THE RELEASE OF ENDOGENOUS NOREPINEPHRINE AND DOPAMINE VIA PRESYNAPTIC BETA-1-ADRENOCEPTORS AND BETA-2-ADRENOCEPTORS UNDER ESSENTIALLY COMPLETE INHIBITION OF L-AROMATIC AMINO-ACID DECARBOXYLASE IN RAT HYPOTHALAMIC SLICES [J].

GOSHIMA, Y ;

NAKAMURA, S ;

MISU, Y .

JAPANESE JOURNAL OF PHARMACOLOGY, 1990, 53 (01) :47-56

[7] BIPHASIC ACTIONS OF L-DOPA ON THE RELEASE OF ENDOGENOUS NORADRENALINE AND DOPAMINE FROM RAT HYPOTHALAMIC SLICES [J].

GOSHIMA, Y ;

KUBO, T ;

MISU, Y .

BRITISH JOURNAL OF PHARMACOLOGY, 1986, 89 (01) :229-234

[8] ANTIBODIES TO HORSERADISH-PEROXIDASE AS SPECIFIC NEURONAL MARKERS IN DROSOPHILA AND IN GRASSHOPPER EMBRYOS [J].

JAN, LY ;

JAN, YN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (08) :2700-2704

[9] LOCALIZATION OF TYROSINE HYDROXYLASE-IMMUNOREACTIVE NEURONS IN THE CAT HYPOTHALAMUS, WITH SPECIAL REFERENCE TO FLUORESCENCE HISTOCHEMISTRY [J].

KITAHAMA, K ;

LUPPI, PH ;

BEROD, A ;

GOLDSTEIN, M ;

JOUVET, M .

JOURNAL OF COMPARATIVE NEUROLOGY, 1987, 262 (04) :578-593

[10] ENDOGENOUS L-DOPA, ITS IMMUNOREACTIVITY IN NEURONS OF MIDBRAIN AND ITS PROJECTION FIELDS IN THE CAT [J].

KITAHAMA, K ;

MONS, N ;

OKAMURA, H ;

JOUVET, M ;

GEFFARD, M .

NEUROSCIENCE LETTERS, 1988, 95 (1-3) :47-52