AGROBACTERIUM-MEDIATED TRANSFORMATION OF PEPINO AND REGENERATION OF TRANSGENIC PLANTS

被引：34

作者：

ATKINSON, RG

GARDNER, RC

机构：

[1] UNIV AUCKLAND,CTR GENE TECHNOL,DEPT CELLULAR & MOLEC BIOL,AUCKLAND,NEW ZEALAND

[2] DSIR FRUIT & TREES,AUCKLAND,NEW ZEALAND

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 04期

关键词：

AGROBACTERIUM-MEDIATED TRANSFORMATION; PEPINO; PKIW1110; REGENERATION; SOLANUM-MURICATUM;

D O I：

10.1007/BF00234297

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (beta-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.

引用

页码：208 / 212

页数：5

共 21 条

[1]

ATKINSON RG, 1990, NEW ZEAL J CROP HORT, V18, P153

[2]

Dawes SN, 1983, PLANT BREEDING NZ, P123

[3] EXPRESSION AND INHERITANCE OF KANAMYCIN RESISTANCE IN A LARGE NUMBER OF TRANSGENIC PETUNIAS GENERATED BY AGROBACTERIUM-MEDIATED TRANSFORMATION [J].