INDUCTION OF CORTICOSPINAL TARGET FINDING BY RELEASE OF A DIFFUSIBLE, CHEMOTROPIC FACTOR IN CERVICAL SPINAL GRAY-MATTER

被引:34
作者
JOOSTEN, EAJ [1 ]
VANDERVEN, PFM [1 ]
HOOIVELD, MHW [1 ]
TENDONKELAAR, HJ [1 ]
机构
[1] CATHOLIC UNIV NIJMEGEN,FAC MED,DEPT CELL BIOL & HISTOL,6500 HB NIJMEGEN,NETHERLANDS
关键词
CHEMOTROPISM; CORTICOSPINAL TRACT; COLLAGEN GEL COCULTURE; INVITRO; GUIDANCE; RAT;
D O I
10.1016/0304-3940(91)90752-F
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The outgrowth of corticospinal tract axons in rat spinal cord primarily occurs during the first postnatal week. Axons originating from a group of layer V pyramidal cell bodies situated in the anterior part of the cerebral sensorimotor cortex project mainly to the cervical gray matter (Joosten et al., Dev. Brain Res., 36 (1987) 121-130). By co-culturing explants of the anterior part of the sensorimotor cortex and of cervical spinal gray matter in 3-D collagen gels, a target-specific directional growth of cortical axons towards the cervical spinal gray explant could be demonstrated. After retrograde filling with the fluorescent tracer 1,1-dioctadecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate (DiI), in vivo as well as in vitro, most of the DiI-labelled cortical neurons were located in layer V of the cortical explant, and were characterized by a pyramidal shape. These data suggest that the cervical spinal gray matter target area becomes innervated by corticospinal axons through the release of a diffusible chemotropic factor.
引用
收藏
页码:25 / 28
页数:4
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