UNSTEADY HEAT-TRANSFER IN CAPILLARY ZONE ELECTROPHORESIS .2. COMPUTER-SIMULATIONS

Unsteady heat transfer in capillary zone electrophoresis in considered. Equations are derived that allow the calculation of the temperature evolution of the buffer under three different modes of electrophoresis: current-, voltage- or power-stabilized. In the power-stabilized mode, the temperature increase is intermediate between those in the current- (lowest) and voltage-stabilized (highest) modes. As the shortest transient time and the smallest temperature rise correspond to the current-stabilized mode of operation, this regime is recommended for temperature-sensitive separations and evaluations of the capillary heat-transfer coefficient, especially in the case of instrumentation-lacking forced cooling.