FACTORS THAT AFFECT THE EXTRACTION OF XYLOGLUCAN FROM THE PRIMARY-CELL WALLS OF SUSPENSION-CULTURED ROSE CELLS

The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37-degrees than at 25-degrees. At 25-degrees, 6M NaOH was the optimal concentration, but it extracted only 25-30% of the xyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6M NaOH at 25-degrees, water extracted more xyloglucan in 24 h than did additional 6M NaOH.