ACTIVATION OF GUINEA-PIG ALVEOLAR MACROPHAGES BY ENDOTHELIN-1

被引：32

作者：

MILLUL, V

LAGENTE, V

GILLARDEAUX, O

BOICHOT, E

DUGAS, B

MENCIAHUERTA, JM

BEREZIAT, G

BRAQUET, P

MASLIAH, J ^{[1
]}

机构：

[1] CHU ST ANTOINE,CNRS,URA 1283,27 RUE CHALIGNY,F-75571 PARIS 12,FRANCE

[2] INST HENRI BEAUFOUR,LES ULIS,FRANCE

来源：

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | 1991年 / 17卷

关键词：

ENDOTHELIN-1; GUINEA PIG ALVEOLAR MACROPHAGES; SUPEROXIDE ANION; ARACHIDONIC ACID; THROMBOXANE;

D O I：

10.1097/00005344-199100177-00067

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Endothelin-1 (ET-1) induced a concentration-and time-dependent increase in arachidonic acid release from guinea pig alveolar macrophages, a maximum being reached at 1 nM ET-1. Regarding the time-course study, maximal release of arachidonic acid was observed after 30 s of incubation with ET-1 and then followed by a marked decrease, suggesting a reuptake of free arachidonic acid. ET-1 (0.1-10 nM) induced a concentration-and time-dependent release of TXB2, the stable metabolite of TXA2, evaluated by an ELISA technique. Maximum release was also obtained with 1 nM ET-1 but only after a 1-min incubation period. By contrast, ET-1 (1 pM-1-mu-M) did not stimulate the release of superoxide anion at any time point of the kinetic study. These results suggest that ET-1 may activate guinea pig alveolar macrophages, probably through a mechanism involving the arachidonic acid pathway.

引用

页码：S233 / S235

页数：3

共 11 条

[1] PHARMACOLOGICAL MODULATION OF THE EFFECTS OF N-FORMYL-L-METHIONYL-L-LEUCYL-L-PHENYLALANINE IN GUINEA-PIGS - INVOLVEMENT OF THE ARACHIDONIC-ACID CASCADE [J].