PEPTIDE SEQUENCING BY MATRIX-ASSISTED LASER-DESORPTION MASS-SPECTROMETRY

被引：275

作者：

SPENGLER, B ^{[1
]}

KIRSCH, D ^{[1
]}

KAUFMANN, R ^{[1
]}

JAEGER, E ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 02期

关键词：

D O I：

10.1002/rcm.1290060207

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method of peptide sequencing by mass spectrometry is described. Metastable decay of laser-desorbed ions, taking place in the first field-free drift region of a reflectron time-of-flight mass spectrometer, has been monitored to get structural information from larger peptides. Fragment ions from metastable decay are mass analysed by adjusting the potentials of the ion reflectron according to the kinetic energies of the ions. The features of the technique and its significance for future applications are outlined.

引用

页码：105 / 108

页数：4

共 10 条

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