LOCALIZATION OF ACTIVIN RECEPTOR (ACTR-IIB2) MESSENGER-RNA IN THE RAT SEMINIFEROUS EPITHELIUM

In situ hybridization was used to localize the mRNA expression of the high affinity activin receptor (ActR-IIB2) in the rat seminiferous epithelium M. ACtR-IIB2 MRNA was expressed maximally in stages IX-XI d the seminferous epithelial cycle. The mRNA signal was detected basally in the epithelium in type A1 and A2 spermatogonia and in Sertoli cells. In the pubertal rat testis the expression was localized in Sertoli cells around primary spermatocytes and around meiotically dividing cells. The localization of ActR-IIB2 mRNA in spermatogonia lends support to the hypothesis that activin is a spermatogonial growth factor. The expression of activin receptor mRNA in pubertal rat testis suggests that activin may have a function during meiotic maturation.