THE USE OF BIOLUMINESCENCE TO STUDY THE BEHAVIOR OF LISTERIA-MONOCYTOGENES WHEN ATTACHED TO SURFACES

被引：12

作者：

WALKER, AJ

HOLAH, JT

DENYER, SP

STEWART, GSAB

机构：

[1] UNIV NOTTINGHAM,DEPT APPL BIOCHEM & FOOD SCI,SUTTON BONINGTON CAMPUS,LOUGHBOROUGH LE12 5RD,LEICS,ENGLAND

[2] CAMPDEN FOOD & DRINK RES ASSOC,DIV MICROBIOL & FOOD HYG,CAMPDEN,GLOS,ENGLAND

[3] UNIV BRIGHTON,DEPT PHARM,BRIGHTON,E SUSSEX,ENGLAND

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 1993年 / 77卷 / 03期

关键词：

BIOFILMS; BIOLUMINESCENCE; LISTERIA-MONOCYTOGENES; PHENOL;

D O I：

10.1016/0927-7757(93)80120-4

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A recombinant derivative of Listeria monocytogenes engineered to express the luxAB genes of Vibrio harveyi has a bioluminescent phenotype that provides a rapid monitor of microbial viability. This phenotype was employed to follow bacterial attachment on to stainless steel surfaces, to monitor the growth of microcolonies upon these surfaces and to assess the resistance of the attached cells following biocide challenge. Real-time bioluminescence measurements provided data equivalent to viable count determinations, and the organism was found to become more resistant to the biocide phenol upon attachment to stainless steel surfaces.

引用

页码：225 / 229

页数：5

共 21 条

[1]

ANWAR H, 1990, ANTIMICROB AGENTS CH, V34, P11

[2]

Characklis W. G., 1990, BIOFILMS, P523

[3] BACTERIAL BIOFILMS IN NATURE AND DISEASE [J].

COSTERTON, JW ;

CHENG, KJ ;

GEESEY, GG ;

LADD, TI ;

NICKEL, JC ;

DASGUPTA, M ;

MARRIE, TJ .

ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :435-464

[4]

DENYER SP, 1992, BIOFOULING, V10, P125

[5] SURFACE-ADHERENT GROWTH OF LISTERIA-MONOCYTOGENES IS ASSOCIATED WITH INCREASED RESISTANCE TO SURFACTANT SANITIZERS AND HEAT [J].

FRANK, JF ;

KOFFI, RA .

JOURNAL OF FOOD PROTECTION, 1990, 53 (07) :550-554

[6] LISTERIOSIS [J].

GELLIN, BG ;

BROOME, CV .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1989, 261 (09) :1313-1320

[7]

HILL PJ, 1993, BIOTECHNOL APPL BIOC, V17, P3

[8] A CONDUCTANCE-BASED SURFACE DISINFECTION TEST FOR FOOD HYGIENE [J].

HOLAH, JT ;

HIGGS, C ;

ROBINSON, S ;

WORTHINGTON, D ;

SPENCELEY, H .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (05) :255-259

[9]

HOLAH JT, 1988, J APPL BACTERIOL, V62, P215

[10]

HOLAH JT, 1992, BIOFILMS SCI TECHNOL, P223

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