PRODUCTION OF GLUCOSAMINE CONTAINING DISACCHARIDES OF THE LEWIS-A AND LEWIS-X TYPES EMPLOYING GLYCOSIDASES

被引：16

作者：

NILSSON, KGI

ELIASSON, A

LARSSONFOREK, U

机构：

[1] Glycorex AB, Lund

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 07期

关键词：

D O I：

10.1007/BF00130357

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Disaccharide derivatives of interest for inhibition studies and for synthesis of the blood group determinants Lewis-a and Lewis-x were obtained with glycosidases as catalysts. Thus, Fuc alpha(1-4)(6-OBn)GlcNH(2) beta SEt and Gal beta 1-3(6-OBn)GlcNH(2) beta-SEt were produced employing (6-OBn)GlcNH(2) beta SEt as acceptor and alpha-L-fucosidase and beta-D-galactosidase, respectively, as catalysts. The phthalimido derivative of lactosamine, Gal beta 1-4GlcNPhth beta SEt, was prepared from lactose employing GlcNPhth beta SEt as the acceptor and a yeast beta-galactosidase as catalyst. The reactions were both regio- and stereospecific, which allowed straightforward production of pure products on a g scale and higher.

引用

页码：717 / 722

页数：6

共 8 条

[1] Bevilacqua M., Annu. Rev. Immunol., 11, pp. 767-790, (1993)
[2] Binkley R.W., Protective Groups, Modern Carbohydrate Chemistry, pp. 113-165, (1988)
[3] Feizi T., Current Opin. Struct. Biol., 3, pp. 701-710, (1993)
[4] Khan S.H., Hindsgaul O., Chemical Synthesis of Oligosaccharides, Molecular Glycobiology, pp. 206-229, (1994)
[5] Karlsson K.-A., Annu. Rev. Biochem., 58, pp. 309-350, (1989)
[6] Nilsson K.G.I., Carbohydr. Res., 167, pp. 95-103, (1987)
[7] Nilsson K.G.I., Use of Glycosidases and Glycosyltransferases in the Synthesis of Complex Oligosaccharides and Their Glycosides, ACS Symposium Series, 466, pp. 51-62, (1991)
[8] Toone E.J., Simon E.S., Bednarski M.D., Whitesides G.M., Tetrahedron Rep., 45, pp. 5365-5389, (1989)

← 1 →