UNDETECTABLE EXPRESSION OF GENOMIC PROGESTERONE-RECEPTOR IN HUMAN SPERMATOZOA

被引：28

作者：

CASTILLA, JA ^{[1
]}

GIL, T ^{[1
]}

MOLINA, J ^{[1
]}

HORTAS, ML ^{[1
]}

RODRIGUEZ, F ^{[1
]}

TORRESMUNOZ, J ^{[1
]}

VERGARA, F ^{[1
]}

HERRUZO, AJ ^{[1
]}

机构：

[1] HOSP VIRGEN NIEVES,CTR MATERNO INFANTIL,DEPT OBSTET & GINECOL,UNIDAD REPROD HUMANA,E-18014 GRANADA,SPAIN

来源：

HUMAN REPRODUCTION | 1995年 / 10卷 / 07期

关键词：

GENOMIC PROGESTERONE RECEPTOR; SPERMATOZOA;

D O I：

10.1093/oxfordjournals.humrep.a136169

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The expression of genomic progesterone receptor in human ejaculated spermatozoa was investigated, Spermatozoa from 10 fertile donors who exhibited normal semen parameters were analysed, Indirect immunofluorescence and an enzyme immunoassay using monoclonal antibodies against genomic progesterone receptor were used, Different types of spermatozoa were studied: fresh, post-swim-up (migrated), capacitated and post-artificial induction of the acrosome reaction by calcium ionophore A23187, Progestin receptor-rich T47D human breast cancer cells were used as a positive control, and progestin receptor-poor MDA-MB-231 human breast carcinoma cells were used as a negative control, Genomic progesterone receptor was not detected in fresh, migrated, capacitated and post-acrosome reaction induction human spermatozoa and MDA-MB-231 cells by either indirect immunofluorescence or enzyme immunoassay. However, in T47D cells a mean concentration of 1043.2 +/- 125.2 fmol genomic progesterone receptor/mg protein was observed by enzyme immunoassay, and indirect immunofluorescence results were positive using both flow cytometry and fluorescence microscopy. These findings suggest that the effect of progesterone on human spermatozoa is not mediated by genomic progesterone receptor.

引用

页码：1757 / 1760

页数：4

共 18 条

[1]

Agthoven TV, 1994, AM J PATHOL, V144, P1238

[2]

BERGQVIST A, 1993, FERTIL STERIL, V60, P63

[3]

BLACKMORE PF, 1990, J BIOL CHEM, V265, P1376

[4]

BLACKMORE PF, 1991, J BIOL CHEM, V266, P18655

[5] CELL-SURFACE LOCALIZATION OF A NOVEL NON-GENOMIC PROGESTERONE-RECEPTOR ON THE HEAD OF HUMAN SPERM [J].

BLACKMORE, PF ;

LATTANZIO, FA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (01) :331-336

[6]

HORWITZ KB, 1983, J BIOL CHEM, V258, P7603

[7]

LAND JA, 1992, FERTIL STERIL, V58, P335

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9]

MENDOZA C, 1992, J REPROD FERTIL, V95, P755

[10] THE ACROSOME REACTION-INDUCING ACTIVITY OF INDIVIDUAL HUMAN FOLLICULAR-FLUID SAMPLES IS HIGHLY VARIABLE AND IS RELATED TO THE STEROID CONTENT [J].

MORALES, P ;

LLANOS, M ;

GUTIERREZ, G ;

KOHEN, P ;

VIGIL, P ;

VANTMAN, D .

HUMAN REPRODUCTION, 1992, 7 (05) :646-651

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