MODELING AND ANALYSIS OF BIOSPECIFIC ADSORPTION IN A FINITE BATH

A general model is presented and used to predict the dynamic behavior of the adsorption and wash stages of biospecific adsorption (affinity chromatography) in a finite bath. The model accounts for film and diffusional mass transfer resistances as well as for the rates of interaction between adsorbates and ligands. The model is applicable to single and multicomponent biospecific as well as nonspecific adsorption, and the adsorbates may be monovalent or multivalent. The predictions of the model are compared with the experimental data of the adsorption of beta -galactosidase onto immobilized anti- beta -galactosidase.