INVITRO AND INVIVO EFFECTS OF PROTEIN PHOSPHATASE INHIBITORS, MICROCYSTINS AND NODULARIN, ON MOUSE SKIN AND FIBROBLASTS

被引：189

作者：

MATSUSHIMA, R

YOSHIZAWA, S

WATANABE, MF

HARADA, K

FURUSAWA, M

CARMICHAEL, WW

FUJIKI, H

机构：

[1] NATL CANC CTR,RES INST,DIV CANCER PREVENT,CHUO KU,TOKYO 104,JAPAN

[2] TOKYO METROPOLITAN RES LAB PUBL HLTH,TOKYO 160,JAPAN

[3] MEIJO UNIV,FAC PHARM,NAGOYA,AICHI 468,JAPAN

[4] DAIICHI SEIYAKU CO LTD,RES INST,TOKYO 134,JAPAN

[5] WRIGHT STATE UNIV,DEPT BIOL SCI,DAYTON,OH 45435

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)91226-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three microcystins, YR, LR and RR and nodularin, all of which are hepatotoxic compounds, inhibited dose-dependently the activity of protein phosphatase 2A in and the specific [3H]okadaic acid binding to a cytosolic fraction of mouse skin, as strongly as okadaic acid. However, microcystins and nodularin did not induce any effects on mouse skin or primary human fibroblasts. Microinjection of microcystin YR into primary human fibroblasts induced morphological changes which were induced by incubation with okadaic acid. Microcystins and nodularin penetrate into the epithelial cells of mouse skin and human fibroblasts with difficulty, which reflects tissue specificity of the compounds. © 1990.

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页码：867 / 874

页数：8

相关论文

共 14 条

[1] THE STRUCTURE OF CYANOGINOSIN-LA, A CYCLIC HEPTAPEPTIDE TOXIN FROM THE CYANOBACTERIUM MICROCYSTIS-AERUGINOSA [J].

BOTES, DP ;

TUINMAN, AA ;

WESSELS, PL ;

VILJOEN, CC ;

KRUGER, H ;

WILLIAMS, DH ;

SANTIKARN, S ;

SMITH, RJ ;

HAMMOND, SJ .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1984, (10) :2311-2318

[2]

Carmichael W. W., 1998, HDB NATURAL TOXINS, V3, P121

[3] TOXICITY AND PARTIAL STRUCTURE OF A HEPATOTOXIC PEPTIDE PRODUCED BY THE CYANOBACTERIUM NODULARIA-SPUMIGENA MERTENS EMEND L575 FROM NEW-ZEALAND [J].

CARMICHAEL, WW ;

ESCHEDOR, JT ;

PATTERSON, GML ;

MOORE, RE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (09) :2257-2263

[4] ANALYSIS AND PURIFICATION OF TOXIC PEPTIDES FROM CYANOBACTERIA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

HARADA, KI ;

MATSUURA, K ;

SUZUKI, M ;

OKA, H ;

WATANABE, MF ;

OISHI, S ;

DAHLEM, AM ;

BEASLEY, VR ;

CARMICHAEL, WW .

JOURNAL OF CHROMATOGRAPHY, 1988, 448 (02) :275-283

[5] HYPERPHOSPHORYLATION OF N-60, A PROTEIN STRUCTURALLY AND IMMUNOLOGICALLY RELATED TO NUCLEOLIN AFTER TUMOR-PROMOTER TREATMENT [J].

ISSINGER, OG ;

MARTIN, T ;

RICHTER, WW ;

OLSON, M ;

FUJIKI, H .

EMBO JOURNAL, 1988, 7 (06) :1621-1626

[6]

NISHIWAKI S, 1990, IN PRESS BIOCH BIOHY

[7] NODULARIN, MICROCYSTIN, AND THE CONFIGURATION OF ADDA [J].

RINEHART, KL ;

HARADA, K ;

NAMIKOSHI, M ;

CHEN, C ;

HARVIS, CA ;

MUNRO, MHG ;

BLUNT, JW ;

MULLIGAN, PE ;

BEASLEY, VR ;

DAHLEM, AM ;

CARMICHAEL, WW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (25) :8557-8558

[8] APPARENT ACTIVATION OF PROTEIN-KINASES BY OKADAIC ACID CLASS TUMOR PROMOTERS [J].

SASSA, T ;

RICHTER, WW ;

UDA, N ;

SUGANUMA, M ;

SUGURI, H ;

YOSHIZAWA, S ;

HIROTA, M ;

FUJIKI, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (03) :939-944

[9] ATYPICAL PULMONARY THROMBOSIS CAUSED BY A TOXIC CYANOBACTERIAL PEPTIDE [J].

SLATKIN, DN ;

STONER, RD ;

ADAMS, WH ;

KYCIA, JH ;

SIEGELMAN, HW .

SCIENCE, 1983, 220 (4604) :1383-1385

[10] SPECIFIC BINDING OF OKADAIC ACID, A NEW TUMOR PROMOTER IN MOUSE SKIN [J].

SUGANUMA, M ;

SUTTAJIT, M ;

SUGURI, H ;

OJIKA, M ;

YAMADA, K ;

FUJIKI, H .

FEBS LETTERS, 1989, 250 (02) :615-618