FAST TRANSLOCATION OF PHOSPHATIDYLCHOLINE TO THE OUTER-MEMBRANE LEAFLET AFTER ITS SYNTHESIS AT THE INNER MEMBRANE-SURFACE IN HUMAN ERYTHROCYTES

被引:21
作者
ANDRICK, C [1 ]
BRORING, K [1 ]
DEUTICKE, B [1 ]
HAEST, CWM [1 ]
机构
[1] RHEIN WESTFAL TH AACHEN,FAK MED,INST PHYSIOL,PAUWELSSTR,W-5100 AACHEN,GERMANY
关键词
PHOSPHATIDYLCHOLINE; FLIP-FLOP; ACYL-COA; ARGININE; TEMPERATURE DEPENDENCE; (HUMAN ERYTHROCYTE);
D O I
10.1016/0005-2736(91)90307-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The translocation rate of [C-14]phosphatidylcholine to the outer membrane leaflet of human erythrocytes after its primary synthesis from lysophosphatidylcholine by acylation with C-14-labeled oleic or palmitic acid in the inner leaflet has been measured by following the time-dependent increase of cleavability of C-14-labeled phospholipids by external phospholipase A2 (5 min, 37-degrees-C). Immediately after a short acylation time period of 10 min about 20% of the newly synthesized [C-14]phosphatidylcholine are already detectable in the outer leaflet. After an incubation of 1 h at 37-degrees-C following 10 min of acylation the fractions of labeled and native phosphatidylcholine accessible to the lipase are identical, which demonstrates that [C-14]phosphatidylcholine has attained the same asymmetric distribution as its endogenous analogue. The calculated halftime of the outward translocation is about 20 min and its activation energy is low, 30 kJ/mol. Translocation is inhibited by a 5 min treatment with phenylglyoxal following acylation. A fast translocation is not observed for newly synthesized phosphatidylethanolamine. Results suggest a selective, protein-mediated outward translocation of newly synthesized phosphatidylcholine.
引用
收藏
页码:235 / 241
页数:7
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