DNA HOMOLOGY BETWEEN PYRENOPHORA-JAPONICA AND P-TERES

被引:25
作者
CROUS, PW [1 ]
JANSE, BJH [1 ]
TUNBRIDGE, T [1 ]
HOLZ, G [1 ]
机构
[1] UNIV STELLENBOSCH,DEPT MICROBIOL,STELLENBOSCH 7600,SOUTH AFRICA
来源
MYCOLOGICAL RESEARCH | 1995年 / 99卷
关键词
D O I
10.1016/S0953-7562(09)80778-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pyrenophora isolates associated with net- and spot type net blotch of barley were compared with verified isolates of P. teres f. teres, P. teres f. maculata and P. japonica using total DNA banding patterns, morphology, symptomatology and mating studies. DNA of South African and overseas spot- and net-type isolates digested with Hae III and Msp I showed 74-100% similarity. Furthermore, spot- and net-type isolates could be mated to produce viable progeny, indicating P. teres to have two-allele heterothallism. Single-ascospores inoculated on differential barley cultivars produced spot net or mixed symptoms, suggesting that recombination has occurred. Based on the high degree of homology in DNA banding patterns between P. japonica and P. teres f. maculata, as well as similarities in symptom expression on differential cultivars and general morphology, P. japonica is proposed as synonym of P. teres.
引用
收藏
页码:1098 / 1102
页数:5
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