COOPERATIVE TRANSIENT TRAPPING OF PROTONS BY STRUCTURALLY DISTORTED CHLOROPLAST ATPASE - EVIDENCE FOR THE PROTON WELL

被引：6

作者：

GRIWATZ, C ^{[1
]}

JUNGE, W ^{[1
]}

机构：

[1] UNIV OSNABRUCK,FACHBEREICH BIOL CHEM,W-4500 OSNABRUCK,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1101卷 / 02期

关键词：

ATPASE; PROTON; PROTONMOTIVE FORCE; PHOTOSYNTHESIS; (CHLOROPLAST);

D O I：

10.1016/S0005-2728(05)80031-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of membrane-bound ATPase, CF0CF1, was distorted by mild EDTA treatment, but without solubilization of CF1. Distorted CF0CF1 rapidly trapped protons from the lumen side without transport. Trapping required electric driving force, showed very high cooperativity as function of pH and hysteresis as function of time. It is discussed under the concept of a 'proton well'.

引用

页码：244 / 248

页数：5

共 17 条

[1] PROTON CHANNEL OF THE CHLOROPLAST ATP SYNTHASE, CF0 - ITS TIME-AVERAGED SINGLE-CHANNEL CONDUCTANCE AS FUNCTION OF PH, TEMPERATURE, ISOTOPIC AND IONIC MEDIUM COMPOSITION [J].

ALTHOFF, G ;

LILL, H ;

JUNGE, W .

JOURNAL OF MEMBRANE BIOLOGY, 1989, 108 (03) :263-271

[2] SUBUNIT-DELTA OF H+-ATPASES - AT THE INTERFACE BETWEEN PROTON FLOW AND ATP SYNTHESIS [J].

ENGELBRECHT, S ;

JUNGE, W .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1015 (03) :379-390

[3] RECONSTITUTION OF CF1-DEPLETED THYLAKOID MEMBRANES WITH COMPLETE AND FRAGMENTED CHLOROPLAST ATPASE - THE ROLE OF THE DELTA-SUBUNIT FOR PROTON CONDUCTION THROUGH CF0 [J].

ENGELBRECHT, S ;

LILL, H ;

JUNGE, W .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 160 (03) :635-643

[4] ESSENTIAL RESIDUES IN THE POLAR LOOP REGION OF SUBUNIT-C OF ESCHERICHIA-COLI F1F0 ATP SYNTHASE DEFINED BY RANDOM OLIGONUCLEOTIDE-PRIMED MUTAGENESIS [J].

FRAGA, D ;

FILLINGAME, RH .

JOURNAL OF BACTERIOLOGY, 1991, 173 (08) :2639-2643

[5] KINETICS OF PROTON-TRANSPORT-COUPLED ATP SYNTHESIS IN CHLOROPLASTS - ACTIVATION OF THE ATPASE BY AN ARTIFICIALLY GENERATED DELTA-PH AND DELTA-PSI [J].

GRABER, P ;

JUNESCH, U ;

SCHATZ, GH .

BERICHTE DER BUNSEN-GESELLSCHAFT-PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 1984, 88 (07) :599-608

[6]

HERMOLIN J, 1989, J BIOL CHEM, V264, P3896

[7] THE PHOTOSYNTHETIC WATER OXIDASE - ITS PROTON PUMPING ACTIVITY IS SHORT-CIRCUITED WITHIN THE PROTEIN BY DCCD [J].

JAHNS, P ;

POLLE, A ;

JUNGE, W .

EMBO JOURNAL, 1988, 7 (03) :589-594

[8] INFLUENCE OF THE REDOX STATE AND THE ACTIVATION OF THE CHLOROPLAST ATP SYNTHASE ON PROTON-TRANSPORT-COUPLED ATP SYNTHESIS HYDROLYSIS [J].

JUNESCH, U ;

GRABER, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 893 (02) :275-288

[9] COOPERATIVE TRANSIENT TRAPPING OF PHOTOSYSTEM-II PROTONS BY THE INTEGRAL MEMBRANE PORTION (CF0) OF CHLOROPLAST ATP-SYNTHASE AFTER MILD EXTRACTION OF THE 4-SUBUNIT CATALYTIC PART (CF1) [J].

JUNGE, W ;

HONG, YQ ;

QIAN, LP ;

VIALE, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (10) :3078-3082

[10] BUFFERING CAPACITY OF THE INTERNAL PHASE OF THYLAKOIDS AND THE MAGNITUDE OF THE PH CHANGES INSIDE UNDER FLASHING LIGHT [J].

JUNGE, W ;

AUSLANDER, W ;

MCGEER, AJ ;

RUNGE, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 546 (01) :121-141

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