VALIDATION OF A THEORETICAL-MODEL FOR ADSORPTION USING CEPHALOSPORIN-C AND POLYMERIC REVERSED-PHASE RESINS

In this study a dynamic ''rate-based'' mathematical model of a chromatographic column was solved numerically and validated by the ability to predict breakthrough behavior of cephalosporin C on columns of Amberchrom reversed-phase media. This model accounts for axial dispersion, liquid film mass transfer, pore diffusion coefficient and incorporates a Langmuir non-linear isotherm. The equilibrium isotherm for this system was determined experimentally and shown to follow a Langmuir isotherm. Batch adsorption studies were also performed and used to estimate the value of the pore diffusion coefficient. The model and the parameter values were shown to predict accurately the breakthrough of cephalosporin C on columns of Amberchrom reversed-phase media under different conditions. The validated model was further utilized to study the impact of each system variable on dynamics of cephalosporin C loading onto the column.