NANOPARTICLES AS CARRIERS FOR GROWTH-HORMONE RELEASING-FACTOR

被引：52

作者：

GRANGIER, JL ^{[1
]}

PUYGRENIER, M ^{[1
]}

GAUTIER, JC ^{[1
]}

COUVREUR, P ^{[1
]}

机构：

[1] UNIV PARIS 11,PHARM GALEN LAB,CNRS,UA 1218,RUE JB CLEMENT,F-92296 CHATENAY MALABRY,FRANCE

来源：

JOURNAL OF CONTROLLED RELEASE | 1991年 / 15卷 / 01期

关键词：

NANOPARTICLES; GROWTH HORMONE RELEASING FACTOR; PEPTIDE DRUGS; POLY(ALKYLCYANOACRYLATE); BIODEGRADABLE POLYMERS;

D O I：

10.1016/0168-3659(91)90098-X

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This study presents a useful approach for the identification of factors which must be considered to permit the development of successful nanoparticle growth hormone releasing factor (GRF) formulation. The results show that GRF can be associated with biodegradable poly(alkylcyanoacrylate) nanoparticles. Among other parameters, the moment at which GRF was incorporated in the polymerization medium was found to be a critical factor in avoiding the degradation of GRF and the covalent bonding of the peptide with the polymer. The drug-polymer interaction was, however, confirmed by gel permeation chromatography (GPC), showing the appearance of a heavier polymeric fraction when GRF was added at the very beginning of the polymerization process. Drug-release experiments suggested that the liberation of GRF from nanoparticles resulted rather from the bioerosion of the polymeric matrix than from the passive diffusion of the peptide through the polymer. Indeed, drug-release appeared dependent upon the esterase content of the incubation medium. Finally, it was found that the alkyl chain length of the cyanoacrylic polymer was able to modulate the rate of GRF release.

引用

页码：3 / 13

页数：11

共 16 条

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