THE APPLICATION OF OLIGONUCLEOTIDE PROBES TO HLA CLASS-II TYPING OF THE DRB SUB-REGION

被引：65

作者：

VAUGHAN, RW ^{[1
]}

LANCHBURY, JSS ^{[1
]}

MARSH, SGE ^{[1
]}

HALL, MA ^{[1
]}

BODMER, JG ^{[1
]}

WELSH, KI ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,TISSUE ANTIGEN LAB,LONDON WC2A 3PX,ENGLAND

来源：

TISSUE ANTIGENS | 1990年 / 36卷 / 04期

关键词：

D O I：

10.1111/j.1399-0039.1990.tb01821.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and acccuracy of this scheme are emphasized.

引用

页码：149 / 155

页数：7

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