PROMOTER-SELECTIVE TRANSCRIPTIONAL DEFECT IN CELL-CYCLE MUTANT TS13 RESCUED BY HTAF(II)250

被引:147
作者
WANG, EH
TJIAN, R
机构
[1] Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley
关键词
D O I
10.1126/science.8303298
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The TAF(II)250 subunit of the human transcription factor IID (TFIID) rescues the temperature-sensitive hamster cell line ts13 and overcomes a G1 arrest. Investigation of the transcriptional properties of ts13 nuclear extracts in vitro showed that activation by the site-specific regulators Sp1 and Gal4VP16 is temperature sensitive in ts13 extracts, whereas basal transcription remains unaffected. This transcriptional defect can be rescued by purified human TFIID or by expression of wild-type TAF(II)250 in ts13 cells. Expression from the cyclin A but not c-fos promoter is temperature sensitive in these mutant cells. Thus, the mutation in TAF(II)250 appears to have gene-specific effects that may lead to the ts13 cell cycle phenotype.
引用
收藏
页码:811 / 814
页数:4
相关论文
共 32 条
[1]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[2]   ISOLATION OF COACTIVATORS ASSOCIATED WITH THE TATA-BINDING PROTEIN THAT MEDIATE TRANSCRIPTIONAL ACTIVATION [J].
DYNLACHT, BD ;
HOEY, T ;
TJIAN, R .
CELL, 1991, 66 (03) :563-576
[3]   MULTIPLE PROTEIN-BINDING SITES IN THE 5'-FLANKING REGION REGULATE C-FOS EXPRESSION [J].
GILMAN, MZ ;
WILSON, RN ;
WEINBERG, RA .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (12) :4305-4316
[4]   CYCLIN-A IS REQUIRED FOR THE ONSET OF DNA-REPLICATION IN MAMMALIAN FIBROBLASTS [J].
GIRARD, F ;
STRAUSFELD, U ;
FERNANDEZ, A ;
LAMB, NJC .
CELL, 1991, 67 (06) :1169-1179
[5]   DROSOPHILA TAF(II)40 INTERACTS WITH BOTH A VP16 ACTIVATION DOMAIN AND THE BASAL TRANSCRIPTION FACTOR-TFIIB [J].
GOODRICH, JA ;
HOEY, T ;
THUT, CJ ;
ADMON, A ;
TJIAN, R .
CELL, 1993, 75 (03) :519-530
[6]   THE HUMAN CYTOMEGALO-VIRUS MAJOR IMMEDIATE EARLY PROMOTER CAN BE TRANS-ACTIVATED BY ADENOVIRUS EARLY PROTEINS [J].
GORMAN, CM ;
GIES, D ;
MCCRAY, G ;
HUANG, M .
VIROLOGY, 1989, 171 (02) :377-385
[7]   2 DISTINCT ENHANCERS WITH DIFFERENT CELL SPECIFICITIES COEXIST IN THE REGULATORY REGION OF POLYOMA [J].
HERBOMEL, P ;
BOURACHOT, B ;
YANIV, M .
CELL, 1984, 39 (03) :653-662
[8]  
HIPSKIND RA, 1991, J BIOL CHEM, V266, P19583
[9]   THE P250 SUBUNIT OF NATIVE TATA BOX-BINDING FACTOR-TFIID IS THE CELL-CYCLE REGULATORY PROTEIN-CCG1 [J].
HISATAKE, K ;
HASEGAWA, S ;
TAKADA, R ;
NAKATANI, Y ;
HORIKOSHI, M ;
ROEDER, RG .
NATURE, 1993, 362 (6416) :179-181
[10]   MOLECULAR-CLONING AND FUNCTIONAL-ANALYSIS OF DROSOPHILA TAF110 REVEAL PROPERTIES EXPECTED OF COACTIVATORS [J].
HOEY, T ;
WEINZIERL, ROJ ;
GILL, G ;
CHEN, JL ;
DYNLACHT, BD ;
TJIAN, R .
CELL, 1993, 72 (02) :247-260