A PERIODIC-TABLE OF SYMMETRICAL TANDEM MISMATCHES IN RNA

被引：132

作者：

WU, M ^{[1
]}

MCDOWELL, JA ^{[1
]}

TURNER, DH ^{[1
]}

机构：

[1] UNIV ROCHESTER, DEPT CHEM, ROCHESTER, NY 14627 USA

来源：

BIOCHEMISTRY | 1995年 / 34卷 / 10期

关键词：

D O I：

10.1021/bi00010a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stabilities and structures of a series of RNA octamers containing symmetric tandem mismatches were studied by UV melting and imino proton NMR. The free energy increments for tandem mismatch formation are found to depend upon both mismatch sequence and adjacent base pairs. The observed sequence dependence of tandem mismatch stability is UG/GU > GU/UG > GA/AG greater than or equal to AG/GA > UU/UU > CA/AC greater than or equal to CU/UC similar to UC/CU similar to CC/CC similar to AC/CA similar to AA/AA, and the closing base pair dependence is 5'G/3'C > 5'C/3'G > 5'U/3'A similar to 5'A/3'U. These results differ from expectations based on models used in RNA folding algorithms and from the sequence dependence observed for folding of RNA hairpins.. Imino proton NMR results indicate the sequence dependence is partially due to hydrogen bonding within mismatches.

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页码：3204 / 3211

页数：8

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