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CLONING AND CHARACTERIZATION OF A CDNA-ENCODING THE CYTOSOLIC COPPER ZINC-SUPEROXIDE DISMUTASE FROM SWEET-POTATO TUBEROUS ROOT

被引：25
作者：
LIN, CT
YEH, KW
KAO, MC
SHAW, JF
机构：
[1] NATL TAIWAN UNIV,DEPT BOT,TAIPEI,TAIWAN
[2] NATL DEF MED CTR,DEPT BIOCHEM,TAIPEI,TAIWAN
[3] ACAD SINICA,INST BOT,TAIPEI 115,TAIWAN
来源：
PLANT MOLECULAR BIOLOGY | 1993年 / 23卷 / 04期
关键词：
CDNA; CYTOSOLIC CU ZN-SOD; SUPEROXIDE DISMUTASE; SWEET POTATO; IPOMOEA-BATATAS (L);
D O I：
10.1007/BF00021547
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A full-length cDNA clone encoding a putative copper/zinc-superoxide dismutase (SOD) of sweet potato, Ipomoea batatas (L.) Lam. cv Tainong 57, was isolated from a cDNA library constructed in lambdagt10 from tuber root mRNA. Nucleotide sequence analysis of this cDNA clone revealed that it comprises a complete open reading frame coding for 152 amino acid residues. The deduced amino acid sequence showed higher homology (78-86%) with the sequence of the cytosolic SOD than that of the chloroplast SOD from other plant species. The residues required for coordinating copper and zinc are conserved as they are among all reported Cu/Zn-SOD sequences. In addition, it lacks recognizable plastic or mitochondrial targeting sequences. These data suggest that the isolated sweet potato clone encodes a cytosolic Cu/Zn-SOD.
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页码：911 / 913
页数：3
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