C-13 NUCLEAR-MAGNETIC-RESONANCE AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY STUDIES OF CARBON METABOLISM IN THE ACTINOMYCIN-D PRODUCER STREPTOMYCES-PARVULUS BY USE OF C-13-LABELED PRECURSORS

Fructose and glutamate metabolism was monitored in cell suspensions of Streptomyces parvulus by C-13 nuclear magnetic resonance. The experiments were performed for cells grown with various C-13 sources in a growth medium containing D-[U-C-13]fructose, L-[C-13]glutamate, or L-[U-C-13]aspartate and with nonlabeled precursors to compare intracellular pools in S. parvulus cells at different periods of the cell life cycle. The transport of fructose into the cells was biphasic in nature; during rapid transport, mannitol, fructose, and glucose 6-phosphate were accumulated intracellularly, whereas during the passive diffusion of fructose, the intracellular carbohydrate pool comprised mainly trehalose (1,1'-alpha-alpha-D-glucose). The regulation of fructokinase activity by the intracellular intermediates may play an important role in fructose catabolism in S. parvulus. Transaldolase activity in S. parvulus was determined from the C-13 nuclear magnetic resonance labeling pattern of trehalose carbons obtained from cells grown in medium containing either L-[U-C-13]aspartate or L-[U-C-13]glutamate. Only carbons 4, 5, and 6 of the disaccharide were labeled. Isotopomer analysis of the trehalose carbons led us to conclude that the flux through the reverse glycolytic pathway, condensation of glyceraldehyde 3-phosphate with dihydroxyacetone phosphate, makes at best a minor contribution to the C-13-labeled units observed in trehalose. The pentose pathway and transaldolase activity can explain the labeling pattern of 4,5,6-C-13(3) of trehalose. Moreover, the transfer of the C-13 label of L-[U-C-13]aspartate into the different isotopomers of trehalose C4, C5, and C6 by the transaldolase activity allowed us to calculate the relative fluxes from oxaloacetate via gluconeogenesis and through the tricarboxylic acid cycle. The ratio of the two fluxes is approximately 1. However, the main carbon source for trehalose synthesis in S. parvulus is fructose and not glutamate or aspartate. The C-13 enrichment and isotopomer population, measured by nuclear magnetic resonance and gas chromatography-mass spectrometry, of the actinomycin D peptide ring enabled us to specify the origins of the five amino acids of actinomycin 6. Threonine and proline exhibited isotopomer populations similar to that of the extracellular L-[C-13]glutamate, indicating that protein catabolism is the origin of their C-13 label, whereas the isotopomer populations of sarcosine and N-methylvaline were similar to those of the new intracellular pool of S. parvulus that originated from D-[U-C-13]fructose during the production of actinomycin D.