CHARACTERIZATION OF THE FUNCTIONAL DOMAIN OF TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2)

被引：64

作者：

DECLERCK, YA

YEAN, TD

LEE, Y

TOMICH, JM

LANGLEY, KE

机构：

[1] CHILDRENS HOSP,DIV GENET,LOS ANGELES,CA 90027

[2] AMGEN INC,THOUSAND OAKS,CA 91320

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of the functional domain of tissue inhibitor of metalloproteinases-2 (TIMP-2) was performed using limited proteolytic degradation with trypsin. This treatment generated a 13.5 kDa fragment which was purified and shown to consist of an uncleaved N-terminal region extending from residue 1 to residue 132. The fragment retains the ability to inhibit activated interstitial collagenase and to block the autocatalytic activation of procollagenase.

引用

页码：65 / 69

页数：5

共 32 条

[1] CDNA CLONING AND EXPRESSION OF A METALLOPROTEINASE INHIBITOR RELATED TO TISSUE INHIBITOR OF METALLOPROTEINASES [J].