APOPTOTIC DEATH IN AN IN-VITRO MODEL OF NEURONAL OXIDATIVE STRESS

被引：56

作者：

RATAN, RR

BARABAN, JM

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHYS MED & REHABIL,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21205

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 1995年 / 22卷 / 04期

关键词：

APOPTOSIS; GLUTATHIONE; NEURONS; OXIDATIVE STRESS;

D O I：

10.1111/j.1440-1681.1995.tb02006.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Oxidative stress is believed to be an important mediator of neuronal cell death but the precise mechanism by which this occurs is unknown. 2. We have developed an in vitro model of neuronal oxidative stress to study the pathways by which free radicals kill neurones. 3. We have shown that oxidative stress, cystine deprivation and glutathione depletion results in cell death with the morphological and biochemical features of apoptosis. 4. Neuronal apoptosis induced by oxidative stress can be inhibited by macromolecular synthesis inhibitors. 5. This in vitro model will be a valuable tool for defining the molecular targets of toxic free radicals in neurones and, in turn, in designing rational new therapies for free radical mediated diseases.

引用

页码：309 / 310

页数：2

共 4 条

[1] A NOVEL FUNCTION OF GLUTAMINE IN CELL-CULTURE - UTILIZATION OF GLUTAMINE FOR THE UPTAKE OF CYSTINE IN HUMAN-FIBROBLASTS [J].