PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE MAJOR PERIPHERAL MYELIN GLYCOPROTEIN-PO

被引：15

作者：

BOLLENSEN, E ^{[1
]}

SCHEIDT, P ^{[1
]}

SCHACHNER, M ^{[1
]}

机构：

[1] UNIV HEIDELBERG,DEPT NEUROBIOL,W-6900 HEIDELBERG,GERMANY

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 04期

关键词：

Epitope; L2/HNK‐1; carbohydrate; Monoclonal antibodies; P[!sub]0[!/sub; Peripheral nervous system;

D O I：

10.1111/j.1471-4159.1990.tb01936.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several monoclonal antibodies were generated against the major glycoprotein P0 of human peripheral nervous system myelin. Antibodies were selected for their reactivity with P0 in Western blots. The antibodies were of the immunoglobulin G subclass and reacted with the glycopeptidase F‐treated P0, indicating that the reactive epitope resides in the protein backbone. In fresh frozen and paraffin‐embedded sections of central and peripheral nervous system of rat and human, P0 antibody 592 reacted with myelin sheaths of peripheral, but not central, nervous system. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1110 / 1114

页数：5

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