LOW-COST LIQUID-CHROMATOGRAPHY (LC-LC) .4. PULSED OPEN TUBE GRADIENT GENERATION, A NEW APPROACH FOR GENERATING NANOLITER VOLUME LINEAR AND TAILORED GRADIENTS FOR CAPILLARY ELECTROPHORESIS AND MICRO-LC

There is a need to generate gradients of nanoliter volumes compatible with 25–100 micron i.d. capillaries used in capillary electrophoresis (CE). Balchunas and Sapaniak [1] showed that manually produced step gradients of increasing propanol and surfactant concentration with micellar electrokinetic chromatography gave an improved separation. And while SDS-PAGE has shown remarkable results in capillaries e.g. [2], gradients in gel crosslinking to extend the molecular weight range that can be resolved has not yet been shown in capillaries. Gradients in pH in polyelectrolytes for isoelectric focusing show some of the highest resolution known. These have been generated in-place by. © 1990, Taylor & Francis Group, LLC. All rights reserved.