SELECTIVE UP-REGULATION OF AN NMDA RECEPTOR SUBUNIT MESSENGER-RNA IN CULTURED CEREBELLAR GRANULE CELLS BY K+-INDUCED DEPOLARIZATION AND NMDA TREATMENT

被引：162

作者：

BESSHO, Y ^{[1
]}

NAWA, H ^{[1
]}

NAKANISHI, S ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,BECKMAN NEUROSCI CTR,COLD SPRING HARBOR,NY 11724

来源：

NEURON | 1994年 / 12卷 / 01期

关键词：

D O I：

10.1016/0896-6273(94)90154-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

High KCl or NMDA treatment promotes the survival of cultured neonatal cerebellar granule cells, and these cells become sensitive to NMDA toxicity after prolonged K+ depolarization. Following both treatments, the NMDA receptor increases, as assessed by fura-2 fluorescence analysis of NMDA receptor-mediated intracellular Ca2+ increase. Northern analysis indicates that both treatments specifically up-regulate NMDAR2A subunit mRNA through an increase in resting intracellular Ca2+ concentration. Antisense oligonucleotide analysis further indicates that NMDAR2A mRNA up-regulation is responsible for NMDA receptor induction. Our results demonstrate that regulation of a specific NMDA receptor subunit mRNA governs NMDA receptor induction, which is thought to play an important role in granule cell survival and death.

引用

页码：87 / 95

页数：9

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