TRIDIMENSIONAL ULTRASTRUCTURE OF PERFUSION FIXED GASTROINTESTINAL EPITHELIAL-CELLS BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY

被引:5
作者
KENDALL, CW
RAO, AV
JANEZIC, SA
TEMKIN, RJ
HOLLENBERG, MJ
LEA, PJ
机构
[1] UNIV TORONTO,FAC MED,DEPT NUTR SCI,TORONTO M5S 1A8,ONTARIO,CANADA
[2] UNIV TORONTO,FAC MED,DEPT ANAT,TORONTO M5S 1A8,ONTARIO,CANADA
来源
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE | 1991年 / 18卷 / 03期
关键词
SEM; INTESTINAL MORPHOLOGY; INTRACELLULAR STRUCTURE; MITOCHONDRIA; CELL MEMBRANE;
D O I
10.1002/jemt.1060180304
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Improvements in the design of modern scanning electron microscopes (SEM) and new methods of specimen preparation incorporating chemical removal of the cytosol and cytoskeleton, now make it possible to view cells and their organelles in three dimensions (3D) at high magnification. In this experiment, high resolution SEM (HRSEM) utilizing new methods of tissue preparation was used to study the intracellular structures of the mouse ileum. In addition, in vivo intestinal perfusion was used to further enhance cellular preservation. Using these modifications it was possible to visualize, in 3D, the fine structure of intestinal epithelial cells and intracellular organelles such as the nucleus, mitochondria, endoplasmic reticulum, and Golgi complex, as well as microvili and cell membrane. Whole mitochondria appeared as irregularly shaped organelles which contained tubular cristae. Plate-like cristae were not observed. The brush border was found to be a closely packed array of cylindrical projections. The extensive folding and structural intricacy of lateral cell membranes between absorptive cells could only be appreciated by viewing this tissue with 3D HRSEM. The use of HRSEM to study 3D ultrastructure of cells and their organelles will improve our understanding of the structure-function relationships in both the healthy and diseased gastrointestinal tract.
引用
收藏
页码:223 / 230
页数:8
相关论文
共 27 条
[1]   QUANTITATIVE FREEZE-FRACTURE AND FILIPIN-BINDING STUDY OF RETINAL-PIGMENT EPITHELIAL-CELL BASAL MEMBRANES IN DIABETIC RATS [J].
CALDWELL, RB ;
SLAPNICK, SM ;
MCLAUGHLIN, BJ .
EXPERIMENTAL EYE RESEARCH, 1987, 44 (02) :245-259
[2]   EFFECT OF CHRONIC INTAKE OF DIETARY-FIBERS ON THE ULTRASTRUCTURAL TOPOGRAPHY OF RAT JEJUNUM AND COLON - A SCANNING ELECTRON-MICROSCOPY STUDY [J].
CASSIDY, MM ;
LIGHTFOOT, FG ;
GRAU, LE ;
STORY, JA ;
KRITCHEVSKY, D ;
VAHOUNY, GV .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1981, 34 (02) :218-228
[3]  
CHADWICK VS, 1979, J LAB CLIN MED, V94, P661
[4]  
COOKE GC, 1968, J PATHOL, V98, P157
[5]  
CREAMER B, 1974, SMALL INTESTINE, P1
[6]   ULTRASTRUCTURAL IMMUNOCYTOCHEMICAL LOCALIZATION OF LYSOZYME IN PANETH CELLS OF MAN [J].
ERLANDSEN, SL ;
PARSONS, JA ;
TAYLOR, TD .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1974, 22 (06) :401-413
[7]   PREPARATION OF VASCULAR ENDOTHELIUM FOR SCANNING ELECTRON-MICROSCOPY - COMPARISON OF EFFECTS OF PERFUSION AND IMMERSION FIXATION [J].
GERTZ, SD ;
RENNELS, ML ;
FORBES, MS ;
NELSON, E .
JOURNAL OF MICROSCOPY-OXFORD, 1975, 105 (DEC) :309-313
[8]  
GLAUERT AM, 1974, PRACTICAL METHODS EL, V3, P1
[9]  
HENRY K, 1982, SMALL INTESTINE, P19
[10]   DEEP-ETCH VIEWS OF CLATHRIN ASSEMBLIES [J].
HEUSER, J ;
KIRCHHAUSEN, T .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1985, 92 (1-2) :1-27