12-LIPOXYGENASE FROM RAT BASOPHILIC LEUKEMIA-CELLS - SEPARATION FROM 5-LIPOXYGENASE AND TEMPERATURE-DEPENDENT INACTIVATION BY HYDROPEROXY FATTY-ACID

被引：8

作者：

VANDERDONK, EMM ^{[1
]}

VERHAGEN, J ^{[1
]}

VELDINK, GA ^{[1
]}

VLIEGENTHART, JFG ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT BIOORGAN CHEM,PADUALAAN 8,3584 CH UTRECHT,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1081卷 / 02期

关键词：

12-LIPOXYGENASE; 12-HYDROXYEICOSATETRAENOIC ACID; 13-HYDROXYOCTADECADIENOIC ACID; ENZYME INACTIVATION; HYDROPEROXY FATTY ACID; (RAT BASOPHILIC LEUKEMIA CELL);

D O I：

10.1016/0005-2760(91)90018-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

12-Lipoxygenase and 5-lipoxygenase from rat basophilic leukemia cells were separated by protein-HPLC in a single step. Upon incubation in the presence of Ca2+, 12-lipoxygenase converted arachidonic acid into 12(S)-hydroxyeicosatetraenoic acid and linoleic acid into 13(S)-hydro(pero)xyoctadecadienoic acid. The reaction products were analyzed by reversed-phase and chiral straight-phase HPLC with ultraviolet-detection. Using the cytosolic fraction of rat basophilic leukemia cells, optimal 12-lipoxygenase activity was observed at 10-degrees-C. At 37-degrees-C 12-lipoxygenase was very rapidly inactivated by its own product, hydroperoxy fatty acid, at low concentrations (10-100 nM).

引用

页码：135 / 140

页数：6

共 28 条

[1] KINETIC-STUDIES ON THE INACTIVATION OF 5-LIPOXYGENASE BY 5(S)-HYDROPEROXYEICOSATETRAENOIC ACID [J].