MATHEMATICAL-MODEL DESCRIBING DISPERSION IN FREE SOLUTION CAPILLARY ELECTROPHORESIS UNDER STACKING CONDITIONS

被引：63

作者：

VINTHER, A ^{[1
]}

SOEBERG, H ^{[1
]}

机构：

[1] TECH UNIV DENMARK,DEPT CHEM ENGN,DK-2800 LYNGBY,DENMARK

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 559卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)80055-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

From experiments a mathematical model is derived that quantitatively describes the dispersion processes in free solution capillary electrophoresis (FSCE) under both stacking and non-stacking conditions. The dispersion is subdivided into an axial, a radial and an introduction term. The last term includes an eventual stacking process, extraneous injection and diffusion during sample introduction. Each of the dispersive terms is derived and discussed. Guidelines for improved peak efficiency during FSCE analysis with stacking conditions are presented.

引用

页码：3 / 26

页数：24

共 40 条

[1]

ATKINS PW, 1982, PHYSICAL CHEM

[2]

BUROLLA VP, 1989, AM BIOTECHNOL LA NOV, P12

[3] CAPILLARY ELECTROPHORESIS OF PROTEINS IN BUFFERS CONTAINING HIGH-CONCENTRATIONS OF ZWITTERIONIC SALTS [J].

BUSHEY, MM ;

JORGENSON, JW .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :301-310

[4]

EVERAERTS FM, 1976, ISOTACHOPHORESIS

[5] CAPILLARY ZONE ELECTROPHORESIS - QUANTITATIVE STUDY OF THE EFFECTS OF SOME DISPERSIVE PROCESSES ON THE SEPARATION EFFICIENCY [J].

FORET, F ;

DEML, M ;

BOCEK, P .

JOURNAL OF CHROMATOGRAPHY, 1988, 452 :601-613

[6] CHARACTERIZATION OF HUMAN GROWTH-HORMONE BY CAPILLARY ELECTROPHORESIS [J].

FRENZ, J ;

WU, SL ;

HANCOCK, WS .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :379-391

[7] EARLY PHASES OF THE DISPERSION OF A SAMPLE INJECTED IN POISEUILLE FLOW [J].

GOLAY, MJE ;

ATWOOD, JG .

JOURNAL OF CHROMATOGRAPHY, 1979, 186 (DEC) :353-370

[8] MINIMIZING ADSORPTION OF PROTEINS ON FUSED-SILICA IN CAPILLARY ZONE ELECTROPHORESIS BY THE ADDITION OF ALKALI-METAL SALTS TO THE BUFFERS [J].

GREEN, JS ;

JORGENSON, JW .

JOURNAL OF CHROMATOGRAPHY, 1989, 478 (01) :63-70

[9] A SEMIEMPIRICAL MODEL FOR THE ELECTROPHORETIC MOBILITIES OF PEPTIDES IN FREE-SOLUTION CAPILLARY ELECTROPHORESIS [J].

GROSSMAN, PD ;

COLBURN, JC ;

LAUER, HH .

ANALYTICAL BIOCHEMISTRY, 1989, 179 (01) :28-33

[10] APPLICATION OF FREE-SOLUTION CAPILLARY ELECTROPHORESIS TO THE ANALYTICAL SCALE SEPARATION OF PROTEINS AND PEPTIDES [J].

GROSSMAN, PD ;

COLBURN, JC ;

LAUER, HH ;

NIELSEN, RG ;

RIGGIN, RM ;

SITTAMPALAM, GS ;

RICKARD, EC .

ANALYTICAL CHEMISTRY, 1989, 61 (11) :1186-1194

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