DETERMINATION OF GANGLIOSIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PHOTODIODE-ARRAY DETECTION

被引：5

作者：

PREVITI, M

DOTTA, F

PONTIERI, GM

DIMARIO, U

LENTI, L

机构：

[1] UNIV ROME LA SAPIENZA,DEPT EXPTL MED,VIALE R ELENA 324,I-00161 ROME,ITALY

[2] UNIV ROME LA SAPIENZA,DEPT ENDOCRINOL,I-00185 ROME,ITALY

[3] UNIV ROME LA SAPIENZA,DEPT CLIN & EXPTL MED,I-00185 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 605卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85240-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mixtures of gangliosides were separated by high-performance liquid chromatography (HPLC) on amino-silica columns according to their negative charge and the length of the carbohydrate portion. The use of an on line variable wave-length diode-array detector allows the identification of gangliosides on the basis of their UV spectra with maximum absorbance at 196 nm. Accurate analytical data acquired with the diode-array detector allow the qualitative and quantitative determination of gangliosides and therefore eliminate the need for thin-layer chromatography after HPLC separation.

引用

页码：221 / 225

页数：5

共 8 条

[1] NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF NON-DERIVATIZED GANGLIOSIDE MIXTURES [J].