OPTICAL TRIPLE SENSOR FOR MEASURING PH, OXYGEN AND CARBON-DIOXIDE

被引：76

作者：

WEIGL, BH

HOLOBAR, A

TRETTNAK, W

KLIMANT, I

KRAUS, H

OLEARY, P

WOLFBEIS, OS

机构：

[1] JOANNEUM RES,INST SENSOR INTERFACES,STEYRERGASSE 17,A-8010 GRAZ,AUSTRIA

[2] KARL FRANZENS UNIV,INST ORGAN CHEM,A-8010 GRAZ,AUSTRIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 32卷 / 02期

关键词：

OPTICAL SENSOR; OXYGEN SENSOR; PH SENSOR; CARBON DIOXIDE SENSOR; OPTRODE;

D O I：

10.1016/0168-1656(94)90175-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen and carbon dioxide in bioreactors is presented. The pH and the CO2 sensor are based on the color change of a pH-sensitive dye immobilized on a polymeric support. The resulting changes in absorption are monitored through optical fibers. The oxygen sensor is based on the quenching of the fluorescence of a metal-organic dye. All three sensors are fully LED compatible. The sensitive membranes consist of plastic films and can be stored and replaced conveniently. The sensors are sterilizable with hydrogen peroxide and ethanol. In addition, the pH sensor is steam sterilizable. Accuracy, resolution and reproducibility fulfil the requirements for use in biotechnological applications. Calibration procedures for each sensor are presented. The working principle and the performance of all three sensors are described, with particular emphasis given to their application in bioreactors.

引用

页码：127 / 138

页数：12

共 18 条

[1]

BROWN DE, 1991, CHEM IND, V16, P678

[2] PHOTOPHYSICS AND PHOTOCHEMISTRY OF OXYGEN SENSORS BASED ON LUMINESCENT TRANSITION-METAL COMPLEXES [J].