SCREENING TECHNIQUES FOR DETECTING ALLELIC VARIATION IN DNA-SEQUENCES

被引：145

作者：

LESSA, EP ^{[1
]}

APPLEBAUM, G ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,MUSEUM VERTEBRATE ZOOL,BERKELEY,CA 94720

来源：

MOLECULAR ECOLOGY | 1993年 / 2卷 / 02期

关键词：

ALLELIC VARIATION; DGGE; HETERODUPLEX ANALYSIS; SCREENING TECHNIQUES; SSCP; TGGE;

D O I：

10.1111/j.1365-294X.1993.tb00006.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article reviews four 'DNA screening techniques', namely heteroduplex analysis, single-strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.

引用

页码：119 / 129

页数：11

共 33 条

[1] COMPREHENSIVE DETECTION OF SINGLE BASE CHANGES IN HUMAN GENOMIC DNA USING DENATURING GRADIENT GEL-ELECTROPHORESIS AND A GC CLAMP [J].

ABRAMS, ES ;

MURDAUGH, SE ;

LERMAN, LS .

GENOMICS, 1990, 7 (04) :463-475

[2]

Burke T., 1992, Genes in ecology: the 33rd Symposium of the British Ecological Society, University of East Anglia, Norwich, UK, 1991., P229

[3]

Chamberlain J.S., 1990, PCR PROTOCOLS GUIDE, P272

[4]

CREASE TJ, 1991, MOL BIOL EVOL, V8, P620

[5] NONISOTOPIC DETECTION OF SINGLE-STRAND CONFORMATION POLYMORPHISM (PCR-SSCP) - A RAPID AND SENSITIVE TECHNIQUE IN DIAGNOSIS OF PHENYLKETONURIA [J].

DOCKHORNDWORNICZAK, B ;

DWORNICZAK, B ;

BROMMELKAMP, L ;

BULLES, J ;

HORST, J ;

BOCKER, WW .

NUCLEIC ACIDS RESEARCH, 1991, 19 (09) :2500-2500

[6] HUMAN 28S RIBOSOMAL-RNA SEQUENCE HETEROGENEITY [J].

GONZALEZ, IL ;

SYLVESTER, JE ;

SCHMICKEL, RD .

NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :10213-10224

[7] COMPENSATORY SLIPPAGE IN THE EVOLUTION OF RIBOSOMAL-RNA GENES [J].

HANCOCK, JM ;

DOVER, GA .

NUCLEIC ACIDS RESEARCH, 1990, 18 (20) :5949-5954

[8]

Hayashi K, 1991, PCR Methods Appl, V1, P34

[9]

Hillis D.M., 1990, SCIENCE, V250, P1022

[10]

Hudson R.R., 1990, Oxford Surveys in Evolutionary Biology, V7, P1

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