FUNCTIONAL-ANALYSIS OF THE BACILLUS-SUBTILIS PURT GENE ENCODING FORMATE-DEPENDENT GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE

被引:18
作者
SAXILD, HH [1 ]
JACOBSEN, JH [1 ]
NYGAARD, P [1 ]
机构
[1] UNIV COPENHAGEN, DEPT BIOL CHEM, DK-1307 COPENHAGEN, DENMARK
来源
MICROBIOLOGY-SGM | 1995年 / 141卷
关键词
5'-PHOSPHORIBOSYLGLYCINAMIDE TRANSFORMYLASE; GROWTH PHASE; PURT GENE SEQUENCE; PURT GENE EXPRESSION; PURT-LACZ GENE FUSION;
D O I
10.1099/13500872-141-9-2211
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The purT gene from Bacillus subtilis encoding the formate-dependent glycinamide ribonucleotide transformylase T was cloned by functional complementation of an Escherichia coli purN purT double mutant, The nucleotide sequence revealed an open reading frame of 384 amino acids. The purT amino acid sequence showed similarity to the enzyme phosphoribosylaminoimidazole carboxylase encoded by the purK gene but not to the N-10-formyltetrahydrofolate-dependent glycinamide ribonucleotide transformylase N enzyme encoded by the purN gene, The glycinamide ribonucleotide transformylase T level was repressed in cells grown in rich medium compared to minimal-medium-grown cells. However, when the culture entered the stationary-growth phase the enzyme level increased in rich medium and decreased in minimal medium, By comparing the deduced amino acid sequence of the B. subtilis purT gene product with translated nucleotide sequences in various databanks, evidence for the existence of putative purT genes in the Cram-negative bacteria Pasteurella haemolytica and Pseudomonas aeruginosa was obtained.
引用
收藏
页码:2211 / 2218
页数:8
相关论文
共 33 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]   REGULATION OF BACTERIAL CELL-WALL - ANALYSIS OF A MUTANT OF BACILLUS-SUBTILIS DEFECTIVE IN BIOSYNTHESIS OF TEICHOIC ACID [J].
BOYLAN, RJ ;
BROOKS, D ;
YOUNG, FE ;
MENDELSON, NH .
JOURNAL OF BACTERIOLOGY, 1972, 110 (01) :281-+
[4]   CONSTRUCTION OF A KIT OF REFERENCE STRAINS FOR RAPID GENETIC-MAPPING IN BACILLUS-SUBTILIS-168 [J].
DEDONDER, RA ;
LEPESANT, JA ;
LEPESANTKEJZLAROVA, J ;
BILLAULT, A ;
STEINMETZ, M ;
KUNST, F .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (04) :989-993
[5]   BACILLUS-SUBTILIS PUR OPERON EXPRESSION AND REGULATION [J].
EBBOLE, DJ ;
ZALKIN, H .
JOURNAL OF BACTERIOLOGY, 1989, 171 (04) :2136-2141
[6]  
EBBOLE DJ, 1987, J BIOL CHEM, V262, P8274
[7]   STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].
HANAHAN, D .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580
[8]   THE RIBOSOMAL DATABASE PROJECT [J].
LARSEN, N ;
OLSEN, GJ ;
MAIDAK, BL ;
MCCAUGHEY, MJ ;
OVERBEEK, R ;
MACKE, TJ ;
MARSH, TL ;
WOESE, CR .
NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3021-3023
[9]   MAPPING OF GENE SPECIFYING DNA POLYMERASE-3 OF BACILLUS-SUBTILIS [J].
LOVE, E ;
DAMBROSIO, J ;
BROWN, NC ;
DUBNAU, D .
MOLECULAR & GENERAL GENETICS, 1976, 144 (03) :313-321
[10]   MOLECULAR-CLONING AND CHARACTERIZATION OF GPA1, A G-PROTEIN ALPHA-SUBUNIT GENE FROM ARABIDOPSIS-THALIANA [J].
MA, H ;
YANOFSKY, MF ;
MEYEROWITZ, EM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3821-3825